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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 14 — Jul. 15, 2008
  • pp: 1629–1631

Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator

Reto Fiolka, Markus Beck, and Andreas Stemmer  »View Author Affiliations


Optics Letters, Vol. 33, Issue 14, pp. 1629-1631 (2008)
http://dx.doi.org/10.1364/OL.33.001629


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Abstract

In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.

© 2008 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 10, 2008
Revised Manuscript: June 12, 2008
Manuscript Accepted: June 17, 2008
Published: July 14, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Reto Fiolka, Markus Beck, and Andreas Stemmer, "Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator," Opt. Lett. 33, 1629-1631 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-14-1629


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References

  1. H. Schneckenburger, Curr. Opin. Biotechnol. 16, 13 (2005). [CrossRef] [PubMed]
  2. D. Axelrod, in Biophotonics, Part B, G.Marriot and I.Parker, eds. (Academic, 2003), p. 1. [CrossRef]
  3. W. Lukosz, J. Opt. Soc. Am. 56, 1463 (1966). [CrossRef]
  4. J. T. Frohn, H. F. Knapp, and A. Stemmer, Proc. Natl. Acad. Sci. USA 97, 7232 (2000). [CrossRef] [PubMed]
  5. M. G. L. Gustafsson, J. Microsc. 198, 82 (2000). [CrossRef] [PubMed]
  6. G. Cragg and P. T. C. So, Opt. Lett. 25, 46 (2000). [CrossRef]
  7. A. Sentenac, K. Belkebir, H. Giovannini, and P. C. Chaumet, Opt. Lett. 33, 255 (2008). [CrossRef] [PubMed]
  8. M. Hofmann, C. Eggeling, S. Jakobs, and S. W. Hell, Proc. Natl. Acad. Sci. USA 102, 17565 (2005). [CrossRef] [PubMed]
  9. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, Proc. Natl. Acad. Sci. USA 97, 8206 (2000). [CrossRef] [PubMed]
  10. M. G. L. Gustafsson, Proc. Natl. Acad. Sci. USA 102, 13081 (2005). [CrossRef] [PubMed]
  11. R. Heintzmann, T. M. Jovin, and C. Cremer, J. Opt. Soc. Am. A 19, 1599 (2002). [CrossRef]
  12. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1642 (2006). [CrossRef] [PubMed]
  13. M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006). [CrossRef] [PubMed]
  14. E. Chung, D. K. Kim, and P. T. C. So, Opt. Lett. 31, 945 (2006). [CrossRef] [PubMed]
  15. E. Chung, D. Kim, Y. Cui, Y. H. Kim, and P. T. C. So, Biophys. J. 93, 1747 (2007). [CrossRef] [PubMed]
  16. M. Beck, M. Aschwanden, and A. Stemmer, J. Microsc. (to be published).

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