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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 16 — Aug. 15, 2008
  • pp: 1819–1821

Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy

Daryl Lim, Kengyeh K. Chu, and Jerome Mertz  »View Author Affiliations


Optics Letters, Vol. 33, Issue 16, pp. 1819-1821 (2008)
http://dx.doi.org/10.1364/OL.33.001819


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Abstract

We describe a method of obtaining optical sectioning with a standard wide-field fluorescence microscope. The method involves acquiring two images, one with nonuniform illumination (in our case, speckle) and another with uniform illumination (in our case, randomized speckle). An evaluation of the local contrast in the speckle-illumination image provides an optically sectioned image with low resolution. This is complemented with high-resolution information obtained from the uniform-illumination image. A fusion of both images leads to a full resolution image that is optically sectioned across all spatial frequencies. This hybrid illumination method is fast, robust, and generalizable to a variety of illumination and imaging configurations.

© 2008 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 22, 2008
Manuscript Accepted: June 13, 2008
Published: August 6, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Daryl Lim, Kengyeh K. Chu, and Jerome Mertz, "Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy," Opt. Lett. 33, 1819-1821 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-16-1819


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References

  1. N. Streibl, Optik (Stuttgart) 66, 341 (1984).
  2. J. B. Pawley, Handbook of Biological Confocal Microscopy (Plenum, 2006). [CrossRef]
  3. C. Ventalon and J. Mertz, Opt. Lett. 30, 3350 (2005). [CrossRef]
  4. C. Ventalon and J. Mertz, Opt. Express 14, 7198 (2006). [CrossRef] [PubMed]
  5. C. Ventalon, R. Heintzmann, and J. Mertz, Opt. Lett. 32, 1417 (2007). [CrossRef] [PubMed]
  6. J. W. Goodman, Speckle Phenomena: Theory and Applications (Roberts & Company, 2006).
  7. P. A. Stokseth, J. Opt. Soc. Am. 59, 1314 (1969). [CrossRef]

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