We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.
© 2008 Optical Society of America
Original Manuscript: May 6, 2008
Revised Manuscript: July 23, 2008
Manuscript Accepted: July 24, 2008
Published: September 9, 2008
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics
Niyom Lue, Wonshik Choi, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, and Gabriel Popescu, "Confocal diffraction phase microscopy of live cells," Opt. Lett. 33, 2074-2076 (2008)