OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 18 — Sep. 15, 2008
  • pp: 2164–2166

Hyperspectral in vivo two-photon microscopy of intrinsic contrast

Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, Brenda R. Chen, and Elizabeth M.C. Hillman  »View Author Affiliations


Optics Letters, Vol. 33, Issue 18, pp. 2164-2166 (2008)
http://dx.doi.org/10.1364/OL.33.002164


View Full Text Article

Enhanced HTML    Acrobat PDF (769 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In vivo two-photon imaging of intrinsic contrast can provide valuable information about structural tissue elements such as collagen and elastin and fluorescent metabolites such as nicotinamide adenine dinucleotide. Yet low signal and overlapping emission spectra can make it difficult to identify and delineate these species in vivo. We present a novel approach that combines excitation scanning with spectrally resolved emission two-photon microscopy, allowing distinct structures to be delineated based on their characteristic spectral fingerprints. The amounts of intrinsic fluorophores present in each voxel can also be evaluated. We demonstrate our method using in vivo imaging of nude mouse skin.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Spectroscopy

History
Original Manuscript: April 21, 2008
Revised Manuscript: June 29, 2008
Manuscript Accepted: July 16, 2008
Published: September 12, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, Brenda R. Chen, and Elizabeth M. C. Hillman, "Hyperspectral in vivo two-photon microscopy of intrinsic contrast," Opt. Lett. 33, 2164-2166 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-18-2164


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003). [CrossRef] [PubMed]
  2. S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2811 (2002). [CrossRef] [PubMed]
  3. N. Ramanujam, in Encyclopedia of Analytical Chemistry, R.A.Meyers, ed. (Wiley, 2000), Vol. 1, pp. 20-56.
  4. J. A. Palero, H. S. de Bruijn, A. van der Ploeg-van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen, Opt. Express 14, 4395 (2006). [CrossRef] [PubMed]
  5. J. R. Mansfield, K. W. Gossage, C. C. Hoyt, and R. M. Levenson, J. Biomed. Opt. 10, 041207 (2005). [CrossRef]
  6. A. J. Radosevich, M. B. Bouchard, S. A. Burgess, R. Stolpher, B. Chen, and E. M. C. Hillman, in Biomedical Optics, OSA Technical Digest (CD) (Optical Society of America, 2008), paper BW67.
  7. M. E. Dickinson, E. Simbuerger, B. Zimmermann, C. W. Waters, and S. E. Fraser, J. Biomed. Opt. 8, 329 (2003). [CrossRef] [PubMed]
  8. P. J. Campagnola, H. A. Clark, W. A. Mohler, A. Lewis, and L. M. Loew, J. Biomed. Opt. 6, 277 (2001). [CrossRef] [PubMed]
  9. B. Han, B. Jung, J. S. Nelson, and E.-H. Choi, J. Biomed. Opt. 12, 014006 (2007). [CrossRef] [PubMed]
  10. M. A. Albota, C. Xu, and W. W. Webb, Appl. Opt. 37, 7352 (1998). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

Supplementary Material


» Media 1: AVI (630 KB)     
» Media 2: AVI (2215 KB)     
» Media 3: JPG (988 KB)     
» Media 4: AVI (3662 KB)     

« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited