OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 2 — Jan. 15, 2008
  • pp: 113–115

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging

Egidijus Auksorius, Bosanta R. Boruah, Christopher Dunsby, Peter M.P. Lanigan, Gordon Kennedy, Mark A.A. Neil, and Paul M.W. French  »View Author Affiliations


Optics Letters, Vol. 33, Issue 2, pp. 113-115 (2008)
http://dx.doi.org/10.1364/OL.33.000113


View Full Text Article

Enhanced HTML    Acrobat PDF (247 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate stimulated emission depletion (STED) microscopy implemented in a laser scanning confocal microscope using excitation light derived from supercontinuum generation in a microstructured optical fiber. Images with resolution improvement beyond the far-field diffraction limit in both the lateral and axial directions were acquired by scanning overlapped excitation and depletion beams in two dimensions using the flying spot scanner of a commercially available laser scanning confocal microscope. The spatial properties of the depletion beam were controlled holographically using a programmable spatial light modulator, which can rapidly change between different STED imaging modes and also compensate for aberrations in the optical path. STED fluorescence lifetime imaging microscopy is demonstrated through the use of time-correlated single photon counting.

© 2008 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 19, 2007
Revised Manuscript: November 24, 2007
Manuscript Accepted: November 27, 2007
Published: January 8, 2008

Virtual Issues
Vol. 3, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Egidijus Auksorius, Bosanta R. Boruah, Christopher Dunsby, Peter M. P. Lanigan, Gordon Kennedy, Mark A. A. Neil, and Paul M. W. French, "Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging," Opt. Lett. 33, 113-115 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-2-113


Sort:  Year  |  Journal  |  Reset  

References

  1. S. W. Hell and J. Wichmann, Opt. Lett. 19, 780 (1994). [CrossRef] [PubMed]
  2. T. A. Klar and S. W. Hell, Opt. Lett. 24, 954 (1999). [CrossRef]
  3. G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lurmann, R. Jahn, C. Eggeling, and S. W. Hell, Proc. Natl. Acad. Sci. U.S.A. 103, 11440 (2006). [CrossRef] [PubMed]
  4. K. I. Willig, S. O. Rizzoli, V. Westphal, R. Jahn, and S. W. Hell, Nature 440, 935 (2006). [CrossRef] [PubMed]
  5. B. Treanor, P. M. P. Lanigan, S. Kumar, C. Dunsby, I. Munro, E. Auksorius, F. J. Culley, M. A. Purbhoo, D. Phillips, M. A. A. Neil, D. N. Burshtyn, P. M. W. French, and D. M. Davis, J. Cell Biol. 174, 153 (2006). [CrossRef] [PubMed]
  6. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, Proc. Natl. Acad. Sci. U.S.A. 97, 8206 (2000). [CrossRef] [PubMed]
  7. V. Westphal, C. M. Blanca, M. Dyba, L. Kastrup, and S. W. Hell, Appl. Phys. Lett. 82, 3125 (2003). [CrossRef]
  8. C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, J. Phys. D 37, 3296 (2004). [CrossRef]
  9. M. A. A. Neil, T. Wilson, and R. Juskaitis, J. Microsc. 197, 219 (2000). [CrossRef] [PubMed]
  10. P. Torok and P. R. T. Munro, Opt. Express 12, 3605 (2004). [CrossRef] [PubMed]
  11. B. R. Boruah and M. A. A. Neil, Opt. Express 14, 10377 (2006). [CrossRef] [PubMed]
  12. A. Kudlinski, A. K. George, J. C. Knight, J. C. Travers, A. B. Rulkov, S. V. Popov, and J. R. Taylor, Opt. Express 14, 5715 (2006). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited