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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 2 — Jan. 15, 2008
  • pp: 113–115

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging

Egidijus Auksorius, Bosanta R. Boruah, Christopher Dunsby, Peter M.P. Lanigan, Gordon Kennedy, Mark A.A. Neil, and Paul M.W. French  »View Author Affiliations

Optics Letters, Vol. 33, Issue 2, pp. 113-115 (2008)

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We demonstrate stimulated emission depletion (STED) microscopy implemented in a laser scanning confocal microscope using excitation light derived from supercontinuum generation in a microstructured optical fiber. Images with resolution improvement beyond the far-field diffraction limit in both the lateral and axial directions were acquired by scanning overlapped excitation and depletion beams in two dimensions using the flying spot scanner of a commercially available laser scanning confocal microscope. The spatial properties of the depletion beam were controlled holographically using a programmable spatial light modulator, which can rapidly change between different STED imaging modes and also compensate for aberrations in the optical path. STED fluorescence lifetime imaging microscopy is demonstrated through the use of time-correlated single photon counting.

© 2008 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: October 19, 2007
Revised Manuscript: November 24, 2007
Manuscript Accepted: November 27, 2007
Published: January 8, 2008

Virtual Issues
Vol. 3, Iss. 2 Virtual Journal for Biomedical Optics

Egidijus Auksorius, Bosanta R. Boruah, Christopher Dunsby, Peter M. P. Lanigan, Gordon Kennedy, Mark A. A. Neil, and Paul M. W. French, "Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging," Opt. Lett. 33, 113-115 (2008)

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  1. S. W. Hell and J. Wichmann, Opt. Lett. 19, 780 (1994). [CrossRef] [PubMed]
  2. T. A. Klar and S. W. Hell, Opt. Lett. 24, 954 (1999). [CrossRef]
  3. G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lurmann, R. Jahn, C. Eggeling, and S. W. Hell, Proc. Natl. Acad. Sci. U.S.A. 103, 11440 (2006). [CrossRef] [PubMed]
  4. K. I. Willig, S. O. Rizzoli, V. Westphal, R. Jahn, and S. W. Hell, Nature 440, 935 (2006). [CrossRef] [PubMed]
  5. B. Treanor, P. M. P. Lanigan, S. Kumar, C. Dunsby, I. Munro, E. Auksorius, F. J. Culley, M. A. Purbhoo, D. Phillips, M. A. A. Neil, D. N. Burshtyn, P. M. W. French, and D. M. Davis, J. Cell Biol. 174, 153 (2006). [CrossRef] [PubMed]
  6. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, Proc. Natl. Acad. Sci. U.S.A. 97, 8206 (2000). [CrossRef] [PubMed]
  7. V. Westphal, C. M. Blanca, M. Dyba, L. Kastrup, and S. W. Hell, Appl. Phys. Lett. 82, 3125 (2003). [CrossRef]
  8. C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, J. Phys. D 37, 3296 (2004). [CrossRef]
  9. M. A. A. Neil, T. Wilson, and R. Juskaitis, J. Microsc. 197, 219 (2000). [CrossRef] [PubMed]
  10. P. Torok and P. R. T. Munro, Opt. Express 12, 3605 (2004). [CrossRef] [PubMed]
  11. B. R. Boruah and M. A. A. Neil, Opt. Express 14, 10377 (2006). [CrossRef] [PubMed]
  12. A. Kudlinski, A. K. George, J. C. Knight, J. C. Travers, A. B. Rulkov, S. V. Popov, and J. R. Taylor, Opt. Express 14, 5715 (2006). [CrossRef] [PubMed]

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