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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 2 — Jan. 15, 2008
  • pp: 171–173

Extended depth of focus in tomographic phase microscopy using a propagation algorithm

Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Optics Letters, Vol. 33, Issue 2, pp. 171-173 (2008)
http://dx.doi.org/10.1364/OL.33.000171


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Abstract

Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 μ m from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 μ m . Tomograms with improved focal depth are demonstrated for single HT29 cells.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: November 5, 2007
Revised Manuscript: December 13, 2007
Manuscript Accepted: December 13, 2007
Published: January 10, 2008

Virtual Issues
Vol. 3, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Extended depth of focus in tomographic phase microscopy using a propagation algorithm," Opt. Lett. 33, 171-173 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-2-171

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