OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 20 — Oct. 15, 2008
  • pp: 2365–2367

Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence

Dong Li, Wei Zheng, and Jianan Y. Qu  »View Author Affiliations


Optics Letters, Vol. 33, Issue 20, pp. 2365-2367 (2008)
http://dx.doi.org/10.1364/OL.33.002365


View Full Text Article

Enhanced HTML    Acrobat PDF (283 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A time-resolved spectroscopic imaging system is built to study the fluorescence characteristics of nicotinamide adenine dinucleotide (NADH), an important metabolic coenzyme and endogenous fluorophore in cells. The system provides a unique approach to measure fluorescence signals in different cellular organelles and cytoplasm. The ratios of free over protein-bound NADH signals in cytosol and nucleus are slightly higher than those in mitochondria. The mitochondrial fluorescence contributes about 70% of overall cellular fluorescence and is not a completely dominant signal. Furthermore, NADH signals in mitochondria, cytosol, and the nucleus respond to the changes of cellular activity differently, suggesting that cytosolic and nuclear fluorescence may complicate the well-known relationship between mitochondrial fluorescence and cellular metabolism.

© 2008 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 27, 2008
Revised Manuscript: August 19, 2008
Manuscript Accepted: August 26, 2008
Published: October 14, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Dong Li, Wei Zheng, and Jianan Y. Qu, "Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence," Opt. Lett. 33, 2365-2367 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-20-2365

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited