OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 21 — Nov. 1, 2008
  • pp: 2452–2454

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering

Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, and Adam Wax  »View Author Affiliations

Optics Letters, Vol. 33, Issue 21, pp. 2452-2454 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (190 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(290.1350) Scattering : Backscattering
(290.3200) Scattering : Inverse scattering

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: July 17, 2008
Revised Manuscript: August 27, 2008
Manuscript Accepted: September 8, 2008
Published: October 21, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, and Adam Wax, "Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering," Opt. Lett. 33, 2452-2454 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. A. Wax, J. W. Pyhtila, R. N. Graf, R. Nines, C. W. Boone, R. R. Dasari, M. S. Feld, V. E. Steele, and G. D. Stoner, J. Biomed. Opt. 10, 051604 (2005). [CrossRef] [PubMed]
  2. K. J. Chalut, L. A. Kresty, J. W. Pyhtila, R. Nines, M. Baird, V. E. Steele, and A. Wax, Cancer Epidemiol. Biomarkers Prev. 16, 223 (2007). [CrossRef] [PubMed]
  3. J. W. Pyhtila, R. N. Graf, and A. Wax, Opt. Express 11, 3473 (2003). [CrossRef] [PubMed]
  4. K. J. Chalut, M. G. Giacomelli, and A. Wax, J. Opt. Soc. Am. A 25, 1866 (2008).
  5. K. Chalut, S. Chen, J. Finan, M. Giacomelli, F. Guilak, K. Leong, and A. Wax, Biophys. J. 107, 124107 (2008).
  6. M. I. Mishchenko, L. D. Travis, and D. W. Mackowski, J. Quant. Spectrosc. Radiat. Transf. 55, 535 (1996). [CrossRef]
  7. M. I. Mishchenko, L. D. Travis, and J. W. Hovenier, Light Scattering by Nonspherical Particles: Theory, Measurements and Applications (Academic, 2000).
  8. A. M. K. Nilsson, Appl. Opt. 37, 2735 (1998). [CrossRef]
  9. J. D. Keener, K. J. Chalut, J. W. Pyhtila, and A. Wax, Opt. Lett. 32, 1326 (2007). [CrossRef] [PubMed]
  10. J. R. Mourant, T. Aida, L. Coburn, and J. Ramachandran, Proc. SPIE 5326, 54 (2004). [CrossRef]
  11. D. D. Duncan, and M. E. Thomas, Appl. Opt. 46, 6185 (2007). [CrossRef] [PubMed]
  12. D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 8, 677 (2004). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited