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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 33, Iss. 21 — Nov. 1, 2008
  • pp: 2452–2454

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering

Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, and Adam Wax  »View Author Affiliations


Optics Letters, Vol. 33, Issue 21, pp. 2452-2454 (2008)
http://dx.doi.org/10.1364/OL.33.002452


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Abstract

We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(290.1350) Scattering : Backscattering
(290.3200) Scattering : Inverse scattering

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: July 17, 2008
Revised Manuscript: August 27, 2008
Manuscript Accepted: September 8, 2008
Published: October 21, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, and Adam Wax, "Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering," Opt. Lett. 33, 2452-2454 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-21-2452


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References

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