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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 21 — Nov. 1, 2008
  • pp: 2491–2493

Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source

Brian R. Rankin, Robert R. Kellner, and Stefan W. Hell  »View Author Affiliations


Optics Letters, Vol. 33, Issue 21, pp. 2491-2493 (2008)
http://dx.doi.org/10.1364/OL.33.002491


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Abstract

We describe a subdiffraction-resolution far-field fluorescence microscope employing stimulated emission depletion (STED) with a light source consisting of a microchip laser coupled into a standard single-mode fiber, which, via stimulated Raman scattering (SRS), yields a comb-like spectrum of seven discrete peaks extending from the fundamental wavelength at 532 nm to 620 nm . Each of the spectral peaks can be used as STED light for overcoming the diffraction barrier. This SRS light source enables the simple implementation of multicolor STED and provides a spectral output with multiple available wavelengths from green to red with potential for further expansion.

© 2008 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(290.5910) Scattering : Scattering, stimulated Raman

ToC Category:
Microscopy

History
Original Manuscript: July 31, 2008
Manuscript Accepted: August 27, 2008
Published: October 24, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Brian R. Rankin, Robert R. Kellner, and Stefan W. Hell, "Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source," Opt. Lett. 33, 2491-2493 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-21-2491


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References

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  2. G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, and S. W. Hell, Proc. Natl. Acad. Sci. USA 103, 11440 (2006). [CrossRef] [PubMed]
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