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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 21 — Nov. 1, 2008
  • pp: 2503–2505

Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides

Chaitanya Dongre, Ronald Dekker, Hugo J.W.M. Hoekstra, Markus Pollnau, Rebeca Martinez-Vazquez, Roberto Osellame, Giulio Cerullo, Roberta Ramponi, Rob van Weeghel, Geert A.J. Besselink, and Hans H. van den Vlekkert  »View Author Affiliations

Optics Letters, Vol. 33, Issue 21, pp. 2503-2505 (2008)

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Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 μ m , enabling high-resolution monitoring of the passage of different types of fluorescent analytes when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. Furthermore, we demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled DNA molecules, during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low limit of detection, will enable monitoring of extremely small quantities with high spatial resolution.

© 2008 Optical Society of America

OCIS Codes
(130.3120) Integrated optics : Integrated optics devices
(140.3390) Lasers and laser optics : Laser materials processing
(260.2510) Physical optics : Fluorescence
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(130.2755) Integrated optics : Glass waveguides
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:
Integrated Optics

Original Manuscript: July 18, 2008
Manuscript Accepted: August 22, 2008
Published: October 24, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Chaitanya Dongre, Ronald Dekker, Hugo J. W. M. Hoekstra, Markus Pollnau, Rebeca Martinez-Vazquez, Roberto Osellame, Giulio Cerullo, Roberta Ramponi, Rob van Weeghel, Geert A. J. Besselink, and Hans H. van den Vlekkert, "Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides," Opt. Lett. 33, 2503-2505 (2008)

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