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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 22 — Nov. 15, 2008
  • pp: 2614–2616

Fabrication of holographic diffractive optical elements for enhancing light collection from fluorescence-based biochips

Peter Macko and Maurice P. Whelan  »View Author Affiliations

Optics Letters, Vol. 33, Issue 22, pp. 2614-2616 (2008)

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An approach to enhancing the light collection from fluorescence emitters bound on the surface of a biochip is presented. It is based on the integration of diffractive optical elements on the underside of the chip that are essentially thick volume holograms written into a layer of photopolymer recording material. The high diffractive efficiency and angular selectivity of these types of diffractive elements make them very effective collectors of the spatially anisotropic light emitted by surface-bound fluorophores. The holographic lithography setup used to fabricate the diffractive elements is described. Results obtained using both a focused laser and light from a fluorescence spot to characterize the performance of the diffractive optical elements are presented.

© 2008 Optical Society of America

OCIS Codes
(080.3620) Geometric optics : Lens system design
(090.2890) Holography : Holographic optical elements
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:

Original Manuscript: July 17, 2008
Revised Manuscript: September 9, 2008
Manuscript Accepted: September 22, 2008
Published: November 11, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Peter Macko and Maurice P. Whelan, "Fabrication of holographic diffractive optical elements for enhancing light collection from fluorescence-based biochips," Opt. Lett. 33, 2614-2616 (2008)

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  1. S. R. Weinberger, T. S. Morris, and M. Pawlak, Pharmacogenomics 1, 395 (2000). [CrossRef]
  2. J.-P. Cloarec, Y. Chevolot, E. Laurenceau, M. Phaner-Goutorbe, and E. Souteyrand, ITBM-RBM 29, 105 (2008).
  3. H. Stuart and D. Hall, Phys. Rev. Lett. 80, 5663 (1998). [CrossRef]
  4. Ľ. Polerecký, J. Hamrle, and B. D. MacCraith, Appl. Opt. 39, 3968 (2000). [CrossRef]
  5. J. Enderlein, T. Ruckstuhl, and S. Seeger, Appl. Opt. 38, 724 (1999). [CrossRef]
  6. T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000). [CrossRef] [PubMed]
  7. R. Blue, N. Kent, Ľ. Polerecký, H. McEvoy, D. Gray, and B. D. MacCraith, Electron. Lett. 41, 682 (2005). [CrossRef]
  8. J. R. Krogmeier, I. Schaefer, G. Seward, G. R. Yantz, and J. W. Larson, Lab Chip 7, 1767 (2007). [CrossRef] [PubMed]
  9. F. Baldini, A. Carloni, A. Giannetti, G. Porro, and C. Trono, Anal. Bioanal. Chem. 391, 1837 (2008). [CrossRef] [PubMed]
  10. S. Martin, C. A. Feely, and V. Toal, Appl. Opt. 36, 5757 (1997). [CrossRef] [PubMed]
  11. R. Jallapuram, I. Naydenova, S. Martin, R. G. Howard, V. Toal, S. Frohmann, S. Orlic, and H. J. Eichler, Opt. Mater. 28, 1329 (2006). [CrossRef]
  12. R. J. Collier, C. B. Burckhardt, and L. H. Lin, Optical Holography (Academic, 1971).

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