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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 4 — Feb. 15, 2008
  • pp: 387–389

Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Che-Hang Yu, Shih-Peng Tai, Chun-Ta Kung, Wen-Jeng Lee, Yi-Fan Chan, Hsiang-Lin Liu, Jiun-Yi Lyu, and Chi-Kuang Sun  »View Author Affiliations

Optics Letters, Vol. 33, Issue 4, pp. 387-389 (2008)

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This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.

© 2008 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

Original Manuscript: September 18, 2007
Revised Manuscript: November 21, 2007
Manuscript Accepted: January 7, 2008
Published: February 14, 2008

Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics

Che-Hang Yu, Shih-Peng Tai, Chun-Ta Kung, Wen-Jeng Lee, Yi-Fan Chan, Hsiang-Lin Liu, Jiun-Yi Lyu, and Chi-Kuang Sun, "Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye," Opt. Lett. 33, 387-389 (2008)

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