OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 5 — Mar. 1, 2008
  • pp: 470–472

Comparison of frequency-domain and time-domain fluorescence lifetime tomography

Anand T.N. Kumar, Scott B. Raymond, Brian J. Bacskai, and David A. Boas  »View Author Affiliations

Optics Letters, Vol. 33, Issue 5, pp. 470-472 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (588 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We compare frequency-and time-domain formulations of deep-tissue fluorescence imaging of turbid media. Simulations are used to show that time-domain fluorescence tomography, implemented via the asymptotic lifetime-based approach, offers a significantly better separability of multiple lifetime targets than a frequency-domain approach. We also demonstrate experimentally, using complex-shaped phantoms, the advantages of the asymptotic time-domain approach over a Fourier-based approach for analyzing time-domain fluorescence data.

© 2008 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 4, 2007
Revised Manuscript: January 17, 2008
Manuscript Accepted: January 22, 2008
Published: February 25, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Anand T. N. Kumar, Scott B. Raymond, Brian J. Bacskai, and David A. Boas, "Comparison of frequency-domain and time-domain fluorescence lifetime tomography," Opt. Lett. 33, 470-472 (2008)

Sort:  Year  |  Journal  |  Reset  


  1. J. Wu, L. Perelman, R. R. Dasari, and M. S. Feld, Proc. Natl. Acad. Sci. U.S.A. 94, 8783 (1997). [CrossRef] [PubMed]
  2. S. Bloch, F. Lesage, L. Mackintosh, A. Gandjbakche, K. Liang, and S. Achilefu, J. Biomed. Opt. 10, 054003-1 (2005). [CrossRef]
  3. A. T. N. Kumar, J. Skoch, B. J. Bacskai, D. A. Boas, and A. K. Dunn, Opt. Lett. 30, 3347 (2005). [CrossRef]
  4. A. T. N. Kumar, S. B. Raymond, A. K. Dunn, B. J. Bacskai, and D. A. Boas, "A time domain fluorescence tomography system for small animal imaging," IEEE Trans. Med. Imaging (to be published).
  5. V. Y. Soloviev, J. McGinty, K. B. Tahir, M. A. A. Neil, A. Sardini, J. V. Hajnal, S. R. Arridge, and P. M. W. French, Opt. Lett. 32, 2034 (2007). [CrossRef] [PubMed]
  6. M. A. Oleary, D. A. Boas, X. D. Li, B. Chance, and A. G. Yodh, Opt. Lett. 21, 158 (1996). [CrossRef]
  7. A. B. Milstein, J. J. Stott, S. Oh, D. A. Boas, R. P. Millane, C. A. Bouman, and K. J. Webb, J. Opt. Soc. Am. A 21, 1035 (2004). [CrossRef]
  8. A. Godavarty, E. M. Sevick-Muraca, and M. J. Eppstein, Med. Phys. 32, 992 (2005). [CrossRef] [PubMed]
  9. V. Ntziachristos and R. Weissleder, Opt. Lett. 26, 893 (2001). [CrossRef]
  10. E. E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003). [CrossRef] [PubMed]
  11. R. B. Schultz, J. Ripoll, and V. Ntziachristos, IEEE Trans. Med. Imaging 23, 492 (2004). [CrossRef]
  12. A. T. N. Kumar, S. B. Raymond, G. Boverman, D. A. Boas, and B. J. Bacskai, Opt. Express 14, 12255 (2006). [CrossRef] [PubMed]
  13. P. I. H. Bastiaens and A. Squire, Trends Cell Biol. 9, 48 (1999). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited