OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 7 — Apr. 1, 2008
  • pp: 714–716

Integrated Raman- and angular-scattering microscopy

Zachary J. Smith and Andrew J. Berger  »View Author Affiliations

Optics Letters, Vol. 33, Issue 7, pp. 714-716 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (218 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10 μ m wide. The elastic-scattering signal was analyzed by generalized Lorenz–Mie theory, representing what we believe to be the first experimental validation of the theory’s prediction of angular backscatter from single spheres. The microscope system exhibits 3 nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned.

© 2008 Optical Society of America

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(290.4020) Scattering : Mie theory

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 20, 2007
Revised Manuscript: February 22, 2008
Manuscript Accepted: February 27, 2008
Published: March 28, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Zachary J. Smith and Andrew J. Berger, "Integrated Raman- and angular-scattering microscopy," Opt. Lett. 33, 714-716 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. M. Mannie, T. McConnell, C. Xie, and Y.-Q. Li, J. Immunol. Methods 297, 53 (2005). [CrossRef] [PubMed]
  2. J. Mourant, T. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. Freyer, J. Biomed. Opt. 7, 378 (2002). [CrossRef] [PubMed]
  3. J. Pyhtila and A. Wax, Opt. Express 12, 6178 (2004). [CrossRef]
  4. J. Wilson, C. Bigelow, D. Calkins, and T. Foster, Biophys. J. 88, 2929 (2005). [CrossRef] [PubMed]
  5. W. Cottrell, J. Wilson, and T. Foster, Opt. Lett. 32, 2348 (2007). [CrossRef] [PubMed]
  6. N. Boustany, R. Drezek, and N. Thakor, Biophys. J. 83, 1691 (2002). [CrossRef] [PubMed]
  7. A. Wax, C. Yang, V. Backman, M. Kalashnikov, R. Dasari, and M. Feld, J. Opt. Soc. Am. A 19, 737 (2002). [CrossRef]
  8. H. Fang, L. Qiu, E. Vitkin, M. Zaman, C. Andersson, S. Salahuddin, L. Kimerer, P. Cipolloni, M. Modell, B. Turner, S. Keates, I. Bigio, I. Itzkan, S. Freedman, R. Bansil, E. Hanlon, and L. Perelman, Appl. Opt. 46, 1760 (2007). [CrossRef] [PubMed]
  9. G. Grehan, B. Maheu, and G. Gouesbet, Appl. Opt. 25, 3539 (1986). [CrossRef] [PubMed]
  10. C. Bohren and D. Huffman, Absorption and Scattering by Small Particles (Wiley Interscience, 1983).
  11. J. Lock, Appl. Opt. 34, 559 (1995). [CrossRef] [PubMed]
  12. P. Bevington, Data Reduction and Error Analysis for the Physical Sciences (McGraw-Hill, 1969).
  13. B. Jasse, R. Chao, and L. Koenig, J. Polym. Sci., Polym. Phys. Ed. 16, 2157 (1978). [CrossRef]
  14. C. Xu, P. Carney, and S. Boppart, Opt. Express 13, 5450 (2005). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited