Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium
Optics Letters, Vol. 33, Issue 7, pp. 744-746 (2008)
http://dx.doi.org/10.1364/OL.33.000744
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Abstract
Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye. The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength.
© 2008 Optical Society of America
OCIS Codes
(090.1760) Holography : Computer holography
(120.3940) Instrumentation, measurement, and metrology : Metrology
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1530) Medical optics and biotechnology : Cell analysis
ToC Category:
Holography
History
Original Manuscript: August 22, 2007
Revised Manuscript: February 8, 2008
Manuscript Accepted: March 6, 2008
Published: March 31, 2008
Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Benjamin Rappaz, Florian Charrière, Christian Depeursinge, Pierre J. Magistretti, and Pierre Marquet, "Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium," Opt. Lett. 33, 744-746 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-7-744
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