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Optics Letters

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  • Vol. 33, Iss. 9 — May. 1, 2008
  • pp: 1026–1028

Parallel confocal detection of single molecules in real time

Paul M. Lundquist, Cheng F. Zhong, Peiqian Zhao, Austin B. Tomaney, Paul S. Peluso, John Dixon, Brad Bettman, Yves Lacroix, Deborah P. Kwo, Etienne McCullough, Mark Maxham, Kevin Hester, Paul McNitt, Donald M. Grey, Carlos Henriquez, Mathieu Foquet, Stephen W. Turner, and Denis Zaccarin  »View Author Affiliations


Optics Letters, Vol. 33, Issue 9, pp. 1026-1028 (2008)
http://dx.doi.org/10.1364/OL.33.001026


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Abstract

The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time.

© 2008 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.1790) Microscopy : Confocal microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(350.4238) Other areas of optics : Nanophotonics and photonic crystals
(310.6628) Thin films : Subwavelength structures, nanostructures

ToC Category:
Microscopy

History
Original Manuscript: January 17, 2008
Revised Manuscript: March 6, 2008
Manuscript Accepted: March 14, 2008
Published: April 30, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Paul M. Lundquist, Cheng F. Zhong, Peiqian Zhao, Austin B. Tomaney, Paul S. Peluso, John Dixon, Brad Bettman, Yves Lacroix, Deborah P. Kwo, Etienne McCullough, Mark Maxham, Kevin Hester, Paul McNitt, Donald M. Grey, Carlos Henriquez, Mathieu Foquet, Stephen W. Turner, and Denis Zaccarin, "Parallel confocal detection of single molecules in real time," Opt. Lett. 33, 1026-1028 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-9-1026


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