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Optics Letters

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  • Vol. 33, Iss. 9 — May. 1, 2008
  • pp: 1032–1034

Imaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes

Vassiliy Tsytsarev, Kaushalya Premachandra, Daisuke Takeshita, and Sonya Bahar  »View Author Affiliations


Optics Letters, Vol. 33, Issue 9, pp. 1032-1034 (2008)
http://dx.doi.org/10.1364/OL.33.001032


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Abstract

We applied high-temporal-resolution optical imaging utilizing both the fast intrinsic optical signal (fIOS) and voltage-sensitive dyes (VSDs) to observe the spatiotemporal characteristics of rat somatosensory cortex during electrical stimulation. We find that changes in both the fIOS and VSD signals occur rapidly ( < 30 ms ) after the stimulus is applied, suggesting that both membrane depolarization and transmembrane ion movement occur shortly after the stimulus, preceding the more gradual physiological changes in oxygen consumption revealed by the slower component of the intrinsic optical signal. We find that the VSD signal spreads through a much larger area of cortex than the fIOS.

© 2008 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1420) Medical optics and biotechnology : Biology

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 15, 2008
Revised Manuscript: March 24, 2008
Manuscript Accepted: March 28, 2008
Published: April 30, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Vassiliy Tsytsarev, Kaushalya Premachandra, Daisuke Takeshita, and Sonya Bahar, "Imaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes," Opt. Lett. 33, 1032-1034 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-9-1032


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