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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 34, Iss. 11 — Jun. 1, 2009
  • pp: 1684–1686

Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

Nicolas Olivier, Alexandre Mermillod-Blondin, Craig B. Arnold, and Emmanuel Beaurepaire  »View Author Affiliations


Optics Letters, Vol. 34, Issue 11, pp. 1684-1686 (2009)
http://dx.doi.org/10.1364/OL.34.001684


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Abstract

We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5 × -extended depth-of-field imaging of developing embryos.

© 2009 Optical Society of America

OCIS Codes
(140.3300) Lasers and laser optics : Laser beam shaping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(230.1040) Optical devices : Acousto-optical devices
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

History
Original Manuscript: March 27, 2009
Manuscript Accepted: April 24, 2009
Published: May 28, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Nicolas Olivier, Alexandre Mermillod-Blondin, Craig B. Arnold, and Emmanuel Beaurepaire, "Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens," Opt. Lett. 34, 1684-1686 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-11-1684

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Supplementary Material


» Media 1: AVI (4311 KB)     

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