OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 34, Iss. 11 — Jun. 1, 2009
  • pp: 1684–1686

Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

Nicolas Olivier, Alexandre Mermillod-Blondin, Craig B. Arnold, and Emmanuel Beaurepaire  »View Author Affiliations

Optics Letters, Vol. 34, Issue 11, pp. 1684-1686 (2009)

View Full Text Article

Enhanced HTML    Acrobat PDF (566 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5 × -extended depth-of-field imaging of developing embryos.

© 2009 Optical Society of America

OCIS Codes
(140.3300) Lasers and laser optics : Laser beam shaping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(230.1040) Optical devices : Acousto-optical devices
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

Original Manuscript: March 27, 2009
Manuscript Accepted: April 24, 2009
Published: May 28, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Nicolas Olivier, Alexandre Mermillod-Blondin, Craig B. Arnold, and Emmanuel Beaurepaire, "Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens," Opt. Lett. 34, 1684-1686 (2009)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990). [CrossRef] [PubMed]
  2. J. Durnin, J. J. Miceli, and J. H. Eberly, Phys. Rev. Lett. 58, 1499 (1987). [CrossRef] [PubMed]
  3. D. McGloin and K. Dholakia, Contemp. Phys. 46, 15 (2005). [CrossRef]
  4. P. Dufour, M. Piché, Y. De Koninck, and N. McCarthy, Appl. Opt. 45, 9246 (2006). [CrossRef] [PubMed]
  5. Z. Ding, H. Ren, Y. Zhao, J. S. Nelson, and Z. Chen, Opt. Lett. 27, 243 (2002). [CrossRef]
  6. E. J. Botcherby, R. Juskaitis, and T. Wilson, Opt. Commun. 268, 253 (2006). [CrossRef]
  7. J. A. Davis, C. S. Tuvey, O. Lopez-Coronado, J. Campos, M. J. Yzuel, and C. Iemmi, Opt. Lett. 32, 844 (2007). [CrossRef] [PubMed]
  8. K. A. Higginson, M. A. Costolo, and E. A. Rietman, Appl. Phys. Lett. 84, 843 (2004). [CrossRef]
  9. E. McLeod and C. B. Arnold, J. Appl. Phys. 102, 033104 (2007). [CrossRef]
  10. A. Mermillod-Blondin, E. McLeod, and C. B. Arnold, Opt. Lett. 33, 2146 (2008). [CrossRef] [PubMed]
  11. E. McLeod and C. B. Arnold, Appl. Opt. 47, 3609 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

Supplementary Material

» Media 1: AVI (4311 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited