Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light
Optics Letters, Vol. 34, Issue 12, pp. 1870-1872 (2009)
http://dx.doi.org/10.1364/OL.34.001870
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Abstract
We report a radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for facilitating longitudinally oriented molecules detection. We observe that under tight focusing of radially polarized pump and Stokes light fields with a high-NA objective, RP-CARS radiation from molecules oriented along the longitudinal direction is approximately threefold stronger than that using linearly polarized CARS (LP-CARS) technique. The lateral resolution of RP-CARS imaging can be improved by about 10% compared to the LP-CARS imaging. We demonstrate this RP-CARS technique by imaging the sectioned cottonwood leaf vascular bundles.
© 2009 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.5810) Microscopy : Scanning microscopy
(260.5430) Physical optics : Polarization
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Microscopy
History
Original Manuscript: March 6, 2009
Revised Manuscript: May 11, 2009
Manuscript Accepted: May 12, 2009
Published: June 11, 2009
Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Fake Lu, Wei Zheng, and Zhiwei Huang, "Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light," Opt. Lett. 34, 1870-1872 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-12-1870
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