OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 34, Iss. 13 — Jul. 1, 2009
  • pp: 2066–2068

Feasibility of in vivo imaging of fluorescent proteins using lifetime contrast

Anand T.N. Kumar, Euiheon Chung, Scott B. Raymond, Jeroen A.J.M. van de Water, Khalid Shah, Dai Fukumura, Rakesh K. Jain, Brian J. Bacskai, and David A. Boas  »View Author Affiliations

Optics Letters, Vol. 34, Issue 13, pp. 2066-2068 (2009)

View Full Text Article

Enhanced HTML    Acrobat PDF (238 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We show that fluorescence lifetime is a powerful contrast mechanism that can enhance the whole-body imaging of fluorescent proteins (FPs), in the presence of background tissue autofluorescence (AF). The nonexponential AF decay is characterized from time-domain (TD) measurements on multiple nude mice and separated from the FP fluorescence using a linear fit to a priori basis functions. We illustrate this approach using an orthotopic mouse tumor model of breast adenocarcinoma. We also report that four commonly used FPs show distinct lifetimes, indicating their suitability for in vivo lifetime multiplexing. These results suggest the potential for exploiting fluorescence lifetime for imaging FPs for a variety of whole-body small-animal imaging applications.

© 2009 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 16, 2009
Revised Manuscript: May 8, 2009
Manuscript Accepted: May 20, 2009
Published: June 30, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Anand T. N. Kumar, Euiheon Chung, Scott B. Raymond, Jeroen A. J. M. van de Water, Khalid Shah, Dai Fukumura, Rakesh K. Jain, Brian J. Bacskai, and David A. Boas, "Feasibility of in vivo imaging of fluorescent proteins using lifetime contrast," Opt. Lett. 34, 2066-2068 (2009)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. N. C. Shaner, P. A. Steinbach, and R. Y. Tsien, Nat. Methods 2, 905 (2005). [CrossRef] [PubMed]
  2. R. K. Jain, L. L. Munn, and D. Fukumura, Nat. Rev. Cancer 2, 266 (2002). [CrossRef] [PubMed]
  3. P. I. H. Bastiaens and A. Squire, Trends Cell Biol. 9, 48 (1999). [CrossRef] [PubMed]
  4. M. Yang, L. Lingna, P. Jiang, A. R. Moossa, S. Penman, and R. M. Hoffman, Proc. Natl. Acad. Sci. USA 100, 14259 (2003). [CrossRef] [PubMed]
  5. G. Zacharakis, H. Kambara, H. Shih, J. Ripoll, J. Grimm, Y. Saeki, R. Weissleder, and V. Ntziachristos, Proc. Natl. Acad. Sci. USA 102, 18252 (2005). [CrossRef] [PubMed]
  6. R. M. Hoffman and M. Yang, Nat. Protocols 1, 1429 (2006). [CrossRef]
  7. N. C. Deliolanis, R. Kasmieh, T. Wurdinger, B. A. Tannous, K. Shah, and V. Ntziachristos, J. Biomed. Opt. 13, 044008 (2008). [CrossRef] [PubMed]
  8. J. M. Tam, R. Upadhyay, M. J. Pittet, R. Weissleder, and U. Mahmood, Mol. Imaging 6, 269 (2007). [PubMed]
  9. V. Y. Soloviev, J. McGinty, K. B. Tahir, M. A. A. Neil, A. Sardini, J. V. Hajnal, S. R. Arridge, and P. M. W. French, Opt. Lett. 32, 2034 (2007). [CrossRef] [PubMed]
  10. A. T. N. Kumar, S. B. Raymond, A. K. Dunn, B. J. Bacskai, and D. A. Boas, IEEE Trans. Med. Imaging 27, 1152 (2008). [CrossRef] [PubMed]
  11. A. T. N. Kumar, S. B. Raymond, B. J. Bacskai, and D. A. Boas, Opt. Lett. 33, 470 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited