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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 34, Iss. 13 — Jul. 1, 2009
  • pp: 2081–2083

Fluorescence lifetime imaging microscopy: in vivo application to diagnosis of oral carcinoma

Yinghua Sun, Jennifer Phipps, Daniel S. Elson, Heather Stoy, Steven Tinling, Jeremy Meier, Brian Poirier, Frank S. Chuang, D. Gregory Farwell, and Laura Marcu  »View Author Affiliations


Optics Letters, Vol. 34, Issue 13, pp. 2081-2083 (2009)
http://dx.doi.org/10.1364/OL.34.002081


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Abstract

A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis and validated in vivo in a hamster oral carcinogenesis model. This apparatus allows for the remote image collection via a flexible imaging probe consisting of a gradient index objective lens and a fiber bundle. Tissue autofluorescence (337 nm excitation) was imaged using an intensified CCD with a gate width down to 0.2 ns. We demonstrate a significant contrast in fluorescence lifetime between tumor ( 1.77 ± 0.26   ns ) and normal ( 2.50 ± 0.36   ns ) tissues at 450 nm and an over 80% intensity decrease at 390 nm emission in tumor versus normal areas. The time-resolved images were minimally affected by tissue morphology, endogenous absorbers, and illumination. These results demonstrate the potential of FLIM as an intraoperative diagnostic technique.

© 2009 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Microscopy

History
Original Manuscript: February 11, 2009
Revised Manuscript: May 13, 2009
Manuscript Accepted: May 15, 2009
Published: June 30, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yinghua Sun, Jennifer Phipps, Daniel S. Elson, Heather Stoy, Steven Tinling, Jeremy Meier, Brian Poirier, Frank S. Chuang, D. Gregory Farwell, and Laura Marcu, "Fluorescence lifetime imaging microscopy: in vivo application to diagnosis of oral carcinoma," Opt. Lett. 34, 2081-2083 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-13-2081


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References

  1. R. Richards-Kortum and E. Sevick-Muraca, Annu. Rev. Phys. Chem. 47, 555 (1996). [CrossRef] [PubMed]
  2. J. R. Lakowicz, Principle of Fluorescence Spectroscopy (Springer, 2006). [CrossRef]
  3. D. Elson, J. Requejo-Isidro, I. Munro, F. Reavell, J. Siegel, K. Suhling, P. Tadrous, R. Benninger, P. Lanigan, J. McGinty, C. Talbot, B. Treanor, S. Webb, A. Sandison, A. Wallace, D. Davis, J. Lever, M. Neil, D. Phillips, G. Stamp, and P. French, Photochem. Photobiol. Sci. 3, 795 (2004). [CrossRef] [PubMed]
  4. I. Pavlova, M. Williams, A. El-Naggar, R. Richards-Kortum, and A. Gillenwater, Clin. Cancer Res. 14, 2396 (2008). [CrossRef] [PubMed]
  5. P. V. Butte, B. K. Pikul, A. Hever, W. H. Yong, K. L. Black, and L. Marcu, J. Biomed. Opt. 10, 064026 (2005). [CrossRef]
  6. I. Munro, J. McGinty, N. Galletly, J. Requejo-Isidro, P. M. P. Lanigan, D. S. Elson, C. Dunsby, M. A. A. Neil, M. J. Lever, G. W. H. Stamp, and P. M. W. French, J. Biomed. Opt. 10, 051403 (2005). [CrossRef] [PubMed]
  7. M. C. Skala, K. M. Riching, D. K. Bird, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, P. J. Keely, and N. Ramanujam, J. Biomed. Opt. 12, 024014 (2007). [CrossRef] [PubMed]
  8. J. Mizeret, T. Stepinac, M. Hansroul, A. Studzinski, H. van den Bergh, and G. Wagnieres, Rev. Sci. Instrum. 70, 4689 (1999). [CrossRef]
  9. D. S. Elson, J. A. Jo, and L. Marcu, New J. Phys. 9, 127 (2007). [CrossRef] [PubMed]
  10. Y. Sun, R. Liu, D. S. Elson, C. W. Hollars, J. A. Jo, J. Park, Y. Sun, and L. Marcu, Opt. Lett. 33, 630 (2008). [CrossRef] [PubMed]
  11. C. T. Chen, H. K. Chiang, S. N. Chow, C. Y. Wang, Y. S. Lee, J. C. Tsai, and C. P. Chiang, J. Oral Pathol. Med. 27, 470 (1998). [CrossRef] [PubMed]

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