Imaging of epithelial tissue in vivo based on excitation of multiple endogenous nonlinear optical signals
Optics Letters, Vol. 34, Issue 18, pp. 2853-2855 (2009)
http://dx.doi.org/10.1364/OL.34.002853
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Abstract
We demonstrate an integrated optical microscope to image the living epithelial tissue by simultaneously exciting multiple endogenous nonlinear optical signals. By employing the spectral lifetime detection capability, this technology provides a unique approach to sensing the fine structure, the protein distribution, and the cellular metabolism of epithelial tissue in vivo. In particular, we investigated the two-photon excitation fluorescence (TPEF) of tryptophan, an essential amino acid serving as the building block of protein. Our findings show that the TPEF of cellular tryptophan produces a good contrast to reveal the morphology of the epithelial cell layer, and the contrast can be further enhanced by applying low-concentration acetic acid.
© 2009 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy
(320.6629) Ultrafast optics : Supercontinuum generation
(170.6935) Medical optics and biotechnology : Tissue characterization
ToC Category:
Biomedical Optics and Biotechnology
History
Original Manuscript: June 12, 2009
Manuscript Accepted: July 19, 2009
Published: September 14, 2009
Virtual Issues
Vol. 4, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Dong Li, Wei Zheng, and Jianan Y. Qu, "Imaging of epithelial tissue in vivo based on excitation of multiple endogenous nonlinear optical signals," Opt. Lett. 34, 2853-2855 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-18-2853
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