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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 34, Iss. 20 — Oct. 15, 2009
  • pp: 3145–3147

Dynamic aberration correction for multiharmonic microscopy

Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire  »View Author Affiliations


Optics Letters, Vol. 34, Issue 20, pp. 3145-3147 (2009)
http://dx.doi.org/10.1364/OL.34.003145


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Abstract

We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2 ( 2 N + 1 ) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: July 21, 2009
Revised Manuscript: September 8, 2009
Manuscript Accepted: September 11, 2009
Published: October 8, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire, "Dynamic aberration correction for multiharmonic microscopy," Opt. Lett. 34, 3145-3147 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-20-3145

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Supplementary Material


» Media 1: AVI (13564 KB)     
» Media 2: AVI (4159 KB)     
» Media 3: AVI (9838 KB)     
» Media 4: AVI (3950 KB)     

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