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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 34, Iss. 20 — Oct. 15, 2009
  • pp: 3145–3147

Dynamic aberration correction for multiharmonic microscopy

Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire  »View Author Affiliations

Optics Letters, Vol. 34, Issue 20, pp. 3145-3147 (2009)

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We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2 ( 2 N + 1 ) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:

Original Manuscript: July 21, 2009
Revised Manuscript: September 8, 2009
Manuscript Accepted: September 11, 2009
Published: October 8, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire, "Dynamic aberration correction for multiharmonic microscopy," Opt. Lett. 34, 3145-3147 (2009)

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