Fourier transform second-harmonic generation (SHG) microscopy has been applied to quantitatively compare the information content between SHG images obtained from the forward and backward direction for three tissue types: porcine tendon, sclera, and ear cartilage. Both signal types yield consistent information on the preferred orientation of collagen fibers. For all specimens, the Fourier transform of the forward and backward SHG images produces several overlapping peaks in the magnitude spectrum at various depths into the tissues, indicating that some information present in the forward SHG images can be extracted from the backward SHG images. This study highlights the potential of backward SHG microscopy for medical diagnostics.
© 2009 Optical Society of America
Original Manuscript: September 16, 2009
Revised Manuscript: October 26, 2009
Manuscript Accepted: October 26, 2009
Published: December 2, 2009
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics
Raghu Ambekar Ramachandra Rao, Monal R. Mehta, Scott Leithem, and Kimani C. Toussaint, Jr., "Quantitative analysis of forward and backward second-harmonic images of collagen fibers using Fourier transform second-harmonic-generation microscopy," Opt. Lett. 34, 3779-3781 (2009)