Using variable pupil filters to optimize the resolution in multiphoton and saturable fluorescence confocal microscopy
Optics Letters, Vol. 34, Issue 4, pp. 464-466 (2009)
http://dx.doi.org/10.1364/OL.34.000464
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Abstract
Pupil filters are widely used to improve the resolution of confocal microscopes. We analyze the possibilities of applying them to N-photon microscopy. We find that taking a linear combination of images obtained with several pupil filters can improve the resolution by a factor of N (compared to a conventional microscope). When applied to saturable fluorescence, this technique allows one to observe fluorescent objects with, in principle, unlimited spatial resolution.
© 2009 Optical Society of America
OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Microscopy
History
Original Manuscript: September 16, 2008
Revised Manuscript: November 21, 2008
Manuscript Accepted: December 8, 2008
Published: February 10, 2009
Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Osip Schwartz and Dan Oron, "Using variable pupil filters to optimize the resolution in multiphoton and saturable fluorescence confocal microscopy," Opt. Lett. 34, 464-466 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-4-464
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