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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 34, Iss. 4 — Feb. 15, 2009
  • pp: 464–466

Using variable pupil filters to optimize the resolution in multiphoton and saturable fluorescence confocal microscopy

Osip Schwartz and Dan Oron  »View Author Affiliations


Optics Letters, Vol. 34, Issue 4, pp. 464-466 (2009)
http://dx.doi.org/10.1364/OL.34.000464


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Abstract

Pupil filters are widely used to improve the resolution of confocal microscopes. We analyze the possibilities of applying them to N-photon microscopy. We find that taking a linear combination of images obtained with several pupil filters can improve the resolution by a factor of N (compared to a conventional microscope). When applied to saturable fluorescence, this technique allows one to observe fluorescent objects with, in principle, unlimited spatial resolution.

© 2009 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 16, 2008
Revised Manuscript: November 21, 2008
Manuscript Accepted: December 8, 2008
Published: February 10, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Osip Schwartz and Dan Oron, "Using variable pupil filters to optimize the resolution in multiphoton and saturable fluorescence confocal microscopy," Opt. Lett. 34, 464-466 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-4-464


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