Dual-interference-channel quantitative-phase microscopy of live cell dynamics
Optics Letters, Vol. 34, Issue 6, pp. 767-769 (2009)
http://dx.doi.org/10.1364/OL.34.000767
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Abstract
We introduce and experimentally demonstrate a fast and accurate method for quantitative imaging of the dynamics of live biological cells. Using a dual-channel interferometric setup, two phase-shifted interferograms of nearly transparent biological samples are acquired in a single digital camera exposure and digitally processed into the phase profile of the sample. Since two interferograms of the same sample are acquired simultaneously, most of the common phase noise is eliminated, enabling the visualization of millisecond-scale dynamic biological phenomena with subnanometer optical path length temporal stability.
© 2009 Optical Society of America
OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography
ToC Category:
Holography
History
Original Manuscript: December 16, 2008
Manuscript Accepted: January 16, 2009
Published: March 6, 2009
Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Natan T. Shaked, Matthew T. Rinehart, and Adam Wax, "Dual-interference-channel quantitative-phase microscopy of live cell dynamics," Opt. Lett. 34, 767-769 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-6-767
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