Comparing coherent and spontaneous Raman scattering under biological imaging conditions
Optics Letters, Vol. 34, Issue 6, pp. 773-775 (2009)
http://dx.doi.org/10.1364/OL.34.000773
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Abstract
We compare imaging based on coherent and spontaneous Raman scattering (SpRS) under conditions relevant for biological imaging. Using a broadband laser source, we perform spectral domain imaging of polystyrene beads using coherent Stokes Raman scattering and SpRS and find comparable signal levels. Short interaction lengths, low molecule number, and low incident power all reduce the advantages available with coherent Raman methods. We present calculations to support our measurements.
© 2009 Optical Society of America
OCIS Codes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(300.6450) Spectroscopy : Spectroscopy, Raman
(180.4315) Microscopy : Nonlinear microscopy
(180.5655) Microscopy : Raman microscopy
ToC Category:
Microscopy
History
Original Manuscript: December 8, 2008
Revised Manuscript: January 23, 2009
Manuscript Accepted: January 23, 2009
Published: March 9, 2009
Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Meng Cui, Brandon R. Bachler, and Jennifer P. Ogilvie, "Comparing coherent and spontaneous Raman scattering under biological imaging conditions," Opt. Lett. 34, 773-775 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-6-773
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