We report a scheme for 2D standing-wave total-internal-reflection fluorescence microscopy. Standing-wave patterns are generated by two interfering beams coupled through the objective lens. Period, angular orientation, and phase of standing waves are controlled entirely by acousto-optic deflectors. The lateral resolution improvement of $100\mathrm{nm}$ is combined with an axial selectivity of $<100\mathrm{nm}$ by utilizing an evanescent standing-wave pattern. This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass–water interface.

© 2009 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Phase-shifting interferometer/ellipsometer capable of measuring the complex index of refraction and the surface profile of a test surface (JOSAA)
• Phase-shifting algorithms for nonlinear and spatially nonuniform phase shifts: reply to comment (JOSAA)
• Punctuated quadrature phase-shifting interferometry (OL)
• Robust one-beam interferometer with phase-delay control (OL)
• Optical phase shifting with acousto-optic devices (OL)

Related Conference Papers

• Experimental Studies of Optical Negative-Index Materials
• 1550nm All-Fiber Interferometer For Partial Discharge Detection In Oil-Insulated Power Transformer
• Digital imaging using a high-resolution interferometric wavefront sensor
• Optical Tracking Microscopy and Super-Resolution Imaging of Living Cells Beyond the Diffraction Limit
• Three-Dimensional Super-Resolution Imaging with a Corkscrew Point Spread Function