We demonstrate in situ characterization of a spatially varying polarization state of an optical field at the focus of a scanning third-harmonic generation (THG) optical microscope. Polarization projections are measured by forming THG images of a polystyrene microsphere scanned through the focused beam and combined in a noniterative phase-retrieval algorithm. Controlled spatially varying polarization states are introduced by imaging spatially inhomogeneous polarization distributions constructed with reflective spatial light modulator to the focal plane of a microscope objective.
© 2009 Optical Society of America
Original Manuscript: November 5, 2008
Manuscript Accepted: January 20, 2009
Published: March 30, 2009
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics
Omid Masihzadeh, Philip Schlup, and Randy A. Bartels, "Control and measurement of spatially inhomogeneous polarization distributions in third-harmonic generation microscopy," Opt. Lett. 34, 1090-1092 (2009)