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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 12 — Jun. 15, 2010
  • pp: 2067–2069

Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue

Zijian Guo, Song Hu, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 35, Issue 12, pp. 2067-2069 (2010)

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Optical absorption is closely associated with many physiological important parameters, such as the concentration and oxygen saturation of hemoglobin, and it can be used to quantify the concentrations of nonfluorescent molecules. We propose a method to use acoustic spectra of photoacoustic signals to quantify the absolute optical absorption. This method is self-calibrating and thus insensitive to variations in the optical fluence. Factors such as system bandwidth and acoustic attenuation can affect the quantification but can be canceled by dividing the acoustic spectra measured at two optical wavelengths. Using optical-resolution photoacoustic microscopy, we quantified the absolute optical absorption of black ink samples with various concentrations. We also quantified both the concentration and oxygen saturation of hemoglobin in a live mouse in absolute units.

© 2010 Optical Society of America

OCIS Codes
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(300.1030) Spectroscopy : Absorption

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 9, 2010
Revised Manuscript: May 14, 2010
Manuscript Accepted: May 9, 2010
Published: June 10, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Zijian Guo, Song Hu, and Lihong V. Wang, "Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue," Opt. Lett. 35, 2067-2069 (2010)

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