Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue
Optics Letters, Vol. 35, Issue 12, pp. 2067-2069 (2010)
http://dx.doi.org/10.1364/OL.35.002067
Enhanced HTML 
Acrobat PDF (349 KB)
Abstract
Optical absorption is closely associated with many physiological important parameters, such as the concentration and oxygen saturation of hemoglobin, and it can be used to quantify the concentrations of nonfluorescent molecules. We propose a method to use acoustic spectra of photoacoustic signals to quantify the absolute optical absorption. This method is self-calibrating and thus insensitive to variations in the optical fluence. Factors such as system bandwidth and acoustic attenuation can affect the quantification but can be canceled by dividing the acoustic spectra measured at two optical wavelengths. Using optical-resolution photoacoustic microscopy, we quantified the absolute optical absorption of black ink samples with various concentrations. We also quantified both the concentration and oxygen saturation of hemoglobin in a live mouse in absolute units.
© 2010 Optical Society of America
OCIS Codes
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(300.1030) Spectroscopy : Absorption
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: February 9, 2010
Revised Manuscript: May 14, 2010
Manuscript Accepted: May 9, 2010
Published: June 10, 2010
Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Zijian Guo, Song Hu, and Lihong V. Wang, "Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue," Opt. Lett. 35, 2067-2069 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-12-2067
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Three-dimensional photoacoustic imaging of blood vessels in tissue (OL)
- Photon path distribution in inhomogeneous turbid media: theoretical analysis and a method of calculation (JOSAA)
- Three-dimensional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact (OL)
- Optoacoustic diffraction tomography: analysis of algorithms (JOSAA)
- Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues (AO)
Related Conference Papers
- Scanning thermoacoustic tomograhy in biological tissue
- Tissue Scaffolding Diagnostics with Femtosecond Laser Radiation
- Biomedical OpticsTissue Scaffolding Diagnostics with Femtosecond Laser Radiation
- Tissue Scaffolding Diagnostics with Femtosecond Laser Radiation
- In vivo Functional Imaging Using Photoacoustic Microscopy
« Previous Article | Next Article »
OSA is a member of 