Flow cytometry using spectrally encoded confocal microscopy
Optics Letters, Vol. 35, Issue 13, pp. 2218-2220 (2010)
http://dx.doi.org/10.1364/OL.35.002218
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Abstract
Flow cytometry techniques often rely on detecting fluorescence from single cells flowing through the cross section of a laser beam, providing invaluable information on vast numbers of cells. Such techniques, however, are often limited in their ability to resolve clusters of cells or parallel cell flow through large vessels. We present a confocal imaging technique that images unstained cells flowing in parallel through a wide channel, using spectrally encoded reflectance confocal microscopy that does not require mechanical scanning. Images of red blood cells from our system are compared to conventional transmission microscopy, and imaging of flowing red blood cells in vitro is experimentally demonstrated.
© 2010 Optical Society of America
OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: March 31, 2010
Revised Manuscript: May 31, 2010
Manuscript Accepted: June 6, 2010
Published: June 24, 2010
Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Lior Golan and Dvir Yelin, "Flow cytometry using spectrally encoded confocal microscopy," Opt. Lett. 35, 2218-2220 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-13-2218
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