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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 17 — Sep. 1, 2010
  • pp: 2988–2990

Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping

Wei Cheng, Ximiao Hou, and Fangmao Ye  »View Author Affiliations

Optics Letters, Vol. 35, Issue 17, pp. 2988-2990 (2010)

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A 1064 nm laser is commonly used for biological optical trapping. However, it has the problem of generating reactive oxygen species in the presence of a sensitizer, which leads to photo damage in biological samples. Here we constructed optical tweezers using a tapered amplifier diode laser that operates at 830 nm . Compared to a 1064 nm laser, this laser is friendly to live cells, eliminates photo damage associated with reactive oxygen species, and allows simultaneous two-photon fluorescence imaging of green fluorescent proteins in live mammalian cells. All these advantages could significantly benefit future application of this single molecule technique in biological studies.

© 2010 Optical Society of America

OCIS Codes
(120.4640) Instrumentation, measurement, and metrology : Optical instruments
(140.2020) Lasers and laser optics : Diode lasers
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Lasers and Laser Optics

Original Manuscript: June 2, 2010
Revised Manuscript: July 24, 2010
Manuscript Accepted: August 4, 2010
Published: August 27, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Wei Cheng, Ximiao Hou, and Fangmao Ye, "Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping," Opt. Lett. 35, 2988-2990 (2010)

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