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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 18 — Sep. 15, 2010
  • pp: 3024–3026

In vivo validation of free-space fluorescence tomography using nuclear imaging

Anikitos Garofalakis, Albertine Dubois, Bertrand Kuhnast, Daniel M. Dupont, Isabelle Janssens, Nicolas Mackiewicz, Frédéric Dollé, Bertrand Tavitian, and Frédéric Ducongé  »View Author Affiliations

Optics Letters, Vol. 35, Issue 18, pp. 3024-3026 (2010)

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The performance of small animal photonic imaging has been considerably improved since the development of fluorescence diffuse optical tomography (fDOT), which can reconstruct fluorescent probe distribution inside tissue. However, the quantification capabilities of this new technology are still a topic of debate, especially in comparison to classical nuclear imaging techniques. Here, we present a method to in vivo calibrate the quantity and localization of a probe provided by free-space fDOT (where no plate is compressing the mouse) with positron emission tomography (PET) and x-ray computed tomography, respectively. This methodology allowed us to demonstrate a strong linear correlation ( R 2 = 0.95 ) between fDOT and PET for probe concentrations ranging from 3 nM to 1 μM in a deep-seated organ.

© 2010 Optical Society of America

OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 18, 2010
Revised Manuscript: August 3, 2010
Manuscript Accepted: August 13, 2010
Published: September 2, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Anikitos Garofalakis, Albertine Dubois, Bertrand Kuhnast, Daniel M. Dupont, Isabelle Janssens, Nicolas Mackiewicz, Frédéric Dollé, Bertrand Tavitian, and Frédéric Ducongé, "In vivo validation of free-space fluorescence tomography using nuclear imaging," Opt. Lett. 35, 3024-3026 (2010)

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