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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 35, Iss. 20 — Oct. 15, 2010
  • pp: 3321–3323

Multiple-trap laser tweezers Raman spectroscopy for simultaneous monitoring of the biological dynamics of multiple individual cells

Pengfei Zhang, Lingbo Kong, Peter Setlow, and Yong-qing Li  »View Author Affiliations


Optics Letters, Vol. 35, Issue 20, pp. 3321-3323 (2010)
http://dx.doi.org/10.1364/OL.35.003321


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Abstract

We report the development of a multiple-trap laser tweezers Raman spectroscopy (LTRS) array for simultaneously acquiring Raman spectra of individual cells in physiological environments. This LTRS-array technique was also combined with phase contrast and fluorescence microscopy, allowing measurement of Raman spectra, refractility, and fluorescence images of individual cells with a temporal resolution of ~5 s. As a demonstration, we used this technique to monitor multiple Bacillus cereus spores germinating in a nutrient medium for up to 90 min and observed the kinetics of dipicolinic acid release and uptake of nucleic acid-binding stain molecules during spore germination.

© 2010 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 28, 2010
Revised Manuscript: August 7, 2010
Manuscript Accepted: August 9, 2010
Published: October 7, 2010

Citation
Pengfei Zhang, Lingbo Kong, Peter Setlow, and Yong-qing Li, "Multiple-trap laser tweezers Raman spectroscopy for simultaneous monitoring of the biological dynamics of multiple individual cells," Opt. Lett. 35, 3321-3323 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-20-3321


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