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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 35, Iss. 20 — Oct. 15, 2010
  • pp: 3390–3392

Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro  »View Author Affiliations


Optics Letters, Vol. 35, Issue 20, pp. 3390-3392 (2010)
http://dx.doi.org/10.1364/OL.35.003390


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Abstract

A completely numerical method, named digital self-referencing holography, is described to easily accomplish a quantitative phase microscopy for microfluidic devices by a digital holographic microscope. The approach works through an appropriate numerical manipulation of the retrieved complex wavefront. The self-referencing is obtained by folding the retrieved wavefront in the image plane. The folding operation allows us to obtain the correct phase map by subtracting from the complex region of interest a flat area outside the microfluidic channel. To demonstrate the effectiveness of the method, quantitative phase maps of bovine spermatozoa and in vitro cells are retrieved.

© 2010 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: May 25, 2010
Revised Manuscript: September 10, 2010
Manuscript Accepted: September 16, 2010
Published: October 8, 2010

Citation
G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, "Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction," Opt. Lett. 35, 3390-3392 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-20-3390

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