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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 35, Iss. 20 — Oct. 15, 2010
  • pp: 3444–3446

Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy

Guillaume Labroille, Rajesh S. Pillai, Xavier Solinas, Caroline Boudoux, Nicolas Olivier, Emmanuel Beaurepaire, and Manuel Joffre  »View Author Affiliations


Optics Letters, Vol. 35, Issue 20, pp. 3444-3446 (2010)
http://dx.doi.org/10.1364/OL.35.003444


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Abstract

We demonstrate selective two-photon excited fluorescence microscopy with shaped pulses produced with a simple yet efficient scheme based on dispersive optical components. The pulse train from a broadband oscillator is split into two subtrains that are sent through different amounts of glass. Beam recombination results in pulse-shape switching at a rate of 150 MHz . Time-resolved photon counting detection then provides two simultaneous images resulting from selective two-photon excitation, as demonstrated in a live embryo. Although less versatile than programmable pulse-shaping devices, this novel arrangement significantly improves the performance of selective microscopy using broadband shaped pulses while simplifying the experimental setup.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(320.5540) Ultrafast optics : Pulse shaping
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 29, 2010
Revised Manuscript: August 27, 2010
Manuscript Accepted: August 30, 2010
Published: October 12, 2010

Citation
Guillaume Labroille, Rajesh S. Pillai, Xavier Solinas, Caroline Boudoux, Nicolas Olivier, Emmanuel Beaurepaire, and Manuel Joffre, "Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy," Opt. Lett. 35, 3444-3446 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-20-3444


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References

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