OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 20 — Oct. 15, 2010
  • pp: 3444–3446

Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy

Guillaume Labroille, Rajesh S. Pillai, Xavier Solinas, Caroline Boudoux, Nicolas Olivier, Emmanuel Beaurepaire, and Manuel Joffre  »View Author Affiliations

Optics Letters, Vol. 35, Issue 20, pp. 3444-3446 (2010)

View Full Text Article

Enhanced HTML    Acrobat PDF (557 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate selective two-photon excited fluorescence microscopy with shaped pulses produced with a simple yet efficient scheme based on dispersive optical components. The pulse train from a broadband oscillator is split into two subtrains that are sent through different amounts of glass. Beam recombination results in pulse-shape switching at a rate of 150 MHz . Time-resolved photon counting detection then provides two simultaneous images resulting from selective two-photon excitation, as demonstrated in a live embryo. Although less versatile than programmable pulse-shaping devices, this novel arrangement significantly improves the performance of selective microscopy using broadband shaped pulses while simplifying the experimental setup.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(320.5540) Ultrafast optics : Pulse shaping
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: June 29, 2010
Revised Manuscript: August 27, 2010
Manuscript Accepted: August 30, 2010
Published: October 12, 2010

Guillaume Labroille, Rajesh S. Pillai, Xavier Solinas, Caroline Boudoux, Nicolas Olivier, Emmanuel Beaurepaire, and Manuel Joffre, "Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy," Opt. Lett. 35, 3444-3446 (2010)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. I. Pastirk, J. D. Cruz, K. Walowicz, V. Lozovoy, and M. Dantus, Opt. Express 11, 1695 (2003). [PubMed]
  2. J. D. Cruz, I. Pastirk, M. Comstock, V. Lozovoy, and M. Dantus, Proc. Natl. Acad. Sci. U.S.A. 101, 16996 (2004).
  3. J. P. Ogilvie, D. Debarre, X. Solinas, J.-L. Martin, E. Beaurepaire, and M. Joffre, Opt. Express 14, 759 (2006). [PubMed]
  4. R. S. Pillai, C. Boudoux, G. Labroille, N. Olivier, I. Veilleux, E. Farge, M. Joffre, and E. Beaurepaire, Opt. Express 17, 12741 (2009). [PubMed]
  5. K. Isobe, A. Suda, M. Tanaka, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, Opt. Express 17, 13737 (2009). [PubMed]
  6. D. Meshulach and Y. Silberberg, Nature 396, 239 (1998).
  7. V. Lozovoy, I. Pastirk, K. Walowicz, and M. Dantus, J. Chem. Phys. 118, 3187 (2003).
  8. W. Amir, R. Carriles, E. Hoover, T. Planchon, C. Durfee, and J. Squier, Opt. Lett. 32, 1731 (2007). [PubMed]
  9. R. Carriles, K. Sheetz, E. Hoover, J. Squier, and V. Barzda, Opt. Express 16, 10364 (2008). [PubMed]
  10. E. Chandler, E. Hoover, J. Field, K. Sheetz, W. Amir, R. Carriles, S. Ding, and J. Squier, Appl. Opt. 48, 2067 (2009). [PubMed]
  11. I. Rocha-Mendoza, W. Langbein, P. Watson, and P. Borri, Opt. Lett. 34, 2258 (2009). [PubMed]
  12. W. Langbein, I. Rocha-Mendoza, and P. Borri, Appl. Phys. Lett. 95, 081109 (2009).
  13. J. D. Cruz, I. Pastirk, V. Lozovoy, K. Walowicz, and M. Dantus, J. Phys. Chem. A 108, 53 (2004).
  14. M. Comstock, V. Lozovoy, I. Pastirk, and M. Dantus, Opt. Express 12, 1061 (2004). [PubMed]
  15. V. Lozovoy, B. Xu, J. Shane, and M. Dantus, Phys. Rev. A 74, 041805 (2006).
  16. R. Fork, O. Martinez, and J. Gordon, Opt. Lett. 9, 150 (1984). [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

Supplementary Material

» Media 1: AVI (10974 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited