We demonstrate orientation-sensitive multimodal nonlinear optical polarizing microscopy capable of probing orientational, polar, and biaxial features of mesomorphic ordering in soft matter. This technique achieves simultaneous imaging in broadband coherent anti-Stokes Raman scattering, multiphoton excitation fluorescence, and multiharmonic generation polarizing microscopy modes and is based on the use of a single femtosecond laser and a photonic crystal fiber as sources of the probing light. We show the viability of this technique for mapping of three-dimensional patterns of molecular orientations and show that images obtained in different microscopy modes are consistent with each other.
© 2010 Optical Society of America
Original Manuscript: August 26, 2010
Manuscript Accepted: September 9, 2010
Published: October 12, 2010
Taewoo Lee, Rahul P. Trivedi, and Ivan I. Smalyukh, "Multimodal nonlinear optical polarizing microscopy of long-range molecular order in liquid crystals," Opt. Lett. 35, 3447-3449 (2010)