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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 35, Iss. 23 — Dec. 1, 2010
  • pp: 3862–3864

Saturated Förster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging

Suhui Deng, Jianfang Chen, Qing Huang, Chunhai Fan, and Ya Cheng  »View Author Affiliations


Optics Letters, Vol. 35, Issue 23, pp. 3862-3864 (2010)
http://dx.doi.org/10.1364/OL.35.003862


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Abstract

We theoretically demonstrate that the spatial resolution of stimulated emission depletion (STED) microscopy can be substantially enhanced without increasing the intensity of the STED beam. In our scheme, tiny nanobeads codoped with donor and acceptor molecules are used as fluorescent probes, in which Förster resonance energy transfer (FRET) can occur with an 100 % efficiency between the donors and acceptors. Enhancement of the depletion of acceptors in the nanobeads with the doughnut-shaped depletion beam can lead to an increase of FRET efficiency in the outer area of the excitation spot, which itself is used for deexciting donor molecules and, consequently, enhancing the optical resolution.

© 2010 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(330.6130) Vision, color, and visual optics : Spatial resolution

ToC Category:
Microscopy

History
Original Manuscript: July 12, 2010
Revised Manuscript: October 16, 2010
Manuscript Accepted: October 18, 2010
Published: November 17, 2010

Citation
Suhui Deng, Jianfang Chen, Qing Huang, Chunhai Fan, and Ya Cheng, "Saturated Förster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging," Opt. Lett. 35, 3862-3864 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-23-3862


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