Saturated Förster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging
Optics Letters, Vol. 35, Issue 23, pp. 3862-3864 (2010)
http://dx.doi.org/10.1364/OL.35.003862
Enhanced HTML 
Acrobat PDF (262 KB)
Abstract
We theoretically demonstrate that the spatial resolution of stimulated emission depletion (STED) microscopy can be substantially enhanced without increasing the intensity of the STED beam. In our scheme, tiny nanobeads codoped with donor and acceptor molecules are used as fluorescent probes, in which Förster resonance energy transfer (FRET) can occur with an
© 2010 Optical Society of America
OCIS Codes
(100.6640) Image processing : Superresolution
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(330.6130) Vision, color, and visual optics : Spatial resolution
ToC Category:
Microscopy
History
Original Manuscript: July 12, 2010
Revised Manuscript: October 16, 2010
Manuscript Accepted: October 18, 2010
Published: November 17, 2010
Citation
Suhui Deng, Jianfang Chen, Qing Huang, Chunhai Fan, and Ya Cheng, "Saturated Förster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging," Opt. Lett. 35, 3862-3864 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-23-3862
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Lateral resolution enhancement with standing evanescent waves (OL)
- Far-field fluorescence microscopy beyond the diffraction limit (JOSAA)
- Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development. (OE)
- Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation (OL)
- Superresolved femtosecond laser nanosurgery of cells (BOE)
Related Conference Papers
- Apertureless near-field fluorescence microscope for biological imaging
- Sub-Resolution Axial Localization of Nanoparticles in Fluorescence Microscopy
- Phase Shift Estimation in Structured Illumination Imaging for Lateral Resolution Enhancement
- Three-Dimensional Localization of Nano-Emitters with Nanometer-Level Precision
- Theoretical Limits on Speed, Errors, and Resolution in Microscopy with Switchable Fluorophores
« Previous Article | Next Article »
OSA is a member of 