Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy
Optics Letters, Vol. 35, Issue 23, pp. 4018-4020 (2010)
http://dx.doi.org/10.1364/OL.35.004018
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Abstract
We investigated the feasibility of simultaneously imaging two distinctive molecular contrasts provided by the absorbed photons in biological tissues with a single light source. The molecular contrasts are based on two physical effects induced by the absorbed photons: photoacoustics (PA) and autofluorescence (AF). In an integrated multimodal imaging system, the PA and AF signals were detected by a high-sensitivity ultrasonic transducer and an avalanche photodetector, respectively. The system was tested by imaging ocular tissue samples, including the retinal pigment epithelium and the ciliary body. The acquired images provided information on the spatial distributions of melanin and lipofuscin in these samples.
© 2010 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.5120) Imaging systems : Photoacoustic imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
ToC Category:
Imaging Systems
History
Original Manuscript: June 15, 2010
Revised Manuscript: September 24, 2010
Manuscript Accepted: November 4, 2010
Published: November 24, 2010
Citation
Xiangyang Zhang, Minshan Jiang, Amani A. Fawzi, Xiang Li, K. Kirk Shung, Carmen A. Puliafito, Hao F. Zhang, and Shuliang Jiao, "Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy," Opt. Lett. 35, 4018-4020 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-23-4018
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