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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 23 — Dec. 1, 2010
  • pp: 4066–4068

Optofluidic system for three-dimensional sensing and identification of micro-organisms with digital holographic microscopy

Donghak Shin, Mehdi Daneshpanah, Arun Anand, and Bahram Javidi  »View Author Affiliations

Optics Letters, Vol. 35, Issue 23, pp. 4066-4068 (2010)

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Optofluidic devices offer flexibility for a variety of tasks involving biological specimen. We propose a system for three-dimensional (3D) sensing and identification of biological micro-organisms. This system consists of a microfluidic device along with a digital holographic microscope and relevant statistical recognition algorithms. The microfluidic channel is used to house the micro-organisms, while the holographic microscope and a CCD camera record their digital holograms. The holograms can be computationally reconstructed in 3D using a variety of algorithms, such as the Fresnel transform. Statistical recognition algorithms are used to analyze and identify the micro-organisms from the reconstructed wavefront. Experimental results are presented. Because of computational reconstruction of wavefronts in holographic imaging, this technique offers unique advantages that allow one to image micro-organisms within a deep channel while removing the inherent microfluidic-induced aberration through interferometery.

© 2010 Optical Society of America

OCIS Codes
(100.5010) Image processing : Pattern recognition
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Imaging Systems

Original Manuscript: September 29, 2010
Revised Manuscript: November 1, 2010
Manuscript Accepted: November 1, 2010
Published: November 30, 2010

Donghak Shin, Mehdi Daneshpanah, Arun Anand, and Bahram Javidi, "Optofluidic system for three-dimensional sensing and identification of micro-organisms with digital holographic microscopy," Opt. Lett. 35, 4066-4068 (2010)

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