We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [ Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.
© 2010 Optical Society of America
Original Manuscript: August 27, 2010
Revised Manuscript: November 5, 2010
Manuscript Accepted: November 8, 2010
Published: December 3, 2010
Etienne Shaffer, Corinne Moratal, Pierre Magistretti, Pierre Marquet, and Christian Depeursinge, "Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy," Opt. Lett. 35, 4102-4104 (2010)
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