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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 35, Iss. 24 — Dec. 15, 2010
  • pp: 4139–4141

In vivo label-free photoacoustic microscopy of cell nuclei by excitation of DNA and RNA

Da-Kang Yao, Konstantin Maslov, Kirk K. Shung, Qifa Zhou, and Lihong V. Wang  »View Author Affiliations


Optics Letters, Vol. 35, Issue 24, pp. 4139-4141 (2010)
http://dx.doi.org/10.1364/OL.35.004139


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Abstract

Imaging of cell nuclei plays a critical role in cancer diagnosis and prognosis. To image noninvasively cell nuclei in vivo without staining, we developed UV photoacoustic microscopy (UV-PAM), in which 266 nm wavelength UV light excites unlabeled DNA and RNA in cell nuclei to produce photoacoustic waves. We applied UV-PAM to ex vivo imaging of cell nuclei in a mouse lip and a mouse small intestine and to in vivo imaging of the cell nuclei in the mouse skin. The UV-PAM images of unstained cell nuclei match the optical micrographs of the histologically stained cell nuclei. Given intrinsic optical contrast and high spatial resolution, in vivo label-free UV-PAM has potential for unique biological and clinical application.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: September 21, 2010
Revised Manuscript: November 4, 2010
Manuscript Accepted: November 8, 2010
Published: December 9, 2010

Citation
Da-Kang Yao, Konstantin Maslov, Kirk K. Shung, Qifa Zhou, and Lihong V. Wang, "In vivo label-free photoacoustic microscopy of cell nuclei by excitation of DNA and RNA," Opt. Lett. 35, 4139-4141 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-24-4139


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