OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 35, Iss. 3 — Feb. 1, 2010
  • pp: 426–428

High-speed line-scan confocal imaging of stimulus-evoked intrinsic optical signals in the retina

Yang-Guo Li, Lei Liu, Franklin Amthor, and Xin-Cheng Yao  »View Author Affiliations


Optics Letters, Vol. 35, Issue 3, pp. 426-428 (2010)
http://dx.doi.org/10.1364/OL.35.000426


View Full Text Article

Enhanced HTML    Acrobat PDF (327 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A rapid line-scan confocal imager was developed for functional imaging of the retina. In this imager, an acousto-optic deflector was employed to produce mechanical vibration- and inertia-free light scanning, and a high-speed (68,000 Hz) linear CCD camera was used to achieve subcellular and submillisecond spatiotemporal resolution imaging. Two imaging modalities, i.e., frame-by-frame and line-by-line recording, were validated for the reflected light detection of intrinsic optical signals (IOSs) in visible light stimulus activated frog retinas. Experimental results indicated that fast IOSs were tightly correlated with retinal stimuli and could track visible light flicker stimulus frequency up to at least 2 Hz.

© 2010 Optical Society of America

OCIS Codes
(330.5310) Vision, color, and visual optics : Vision - photoreceptors
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 5, 2009
Revised Manuscript: December 3, 2009
Manuscript Accepted: December 15, 2009
Published: January 29, 2010

Virtual Issues
Vol. 5, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Yang-Guo Li, Lei Liu, Franklin Amthor, and Xin-Cheng Yao, "High-speed line-scan confocal imaging of stimulus-evoked intrinsic optical signals in the retina," Opt. Lett. 35, 426-428 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-3-426


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. D. R. Pepperberg, M. Kahlert, A. Krause, and K. P. Hofmann, Proc. Natl. Acad. Sci. USA 85, 5531 (1988). [CrossRef] [PubMed]
  2. J. L. Schei, M. D. McCluskey, A. J. Foust, X. C. Yao, and D. M. Rector, Neuroimage 40, 1034 (2008). [CrossRef] [PubMed]
  3. D. A. Nelson, S. Krupsky, A. Pollack, E. Aloni, M. Belkin, I. Vanzetta, M. Rosner, and A. Grinvald, Ophthalmic Surg. Lasers Imaging 36, 57 (2005). [PubMed]
  4. M. D. Abramoff, Y. H. Kwon, D. Ts'o, P. Soliz, B. Zimmerman, J. Pokorny, and R. Kardon, Invest. Ophthalmol. Visual Sci. 47, 715 (2006). [CrossRef]
  5. X. C. Yao, Jpn. J. Ophthalmol. 53, 327 (2009). [CrossRef] [PubMed]
  6. Y. B. Zhao and X. C. Yao, Opt. Lett. 33, 342 (2008). [CrossRef] [PubMed]
  7. X. C. Yao and Y. B. Zhao, Opt. Express 16, 12446 (2008). [CrossRef] [PubMed]
  8. R. S. Jonnal, J. Rha, Y. Zhang, B. Cense, W. Gao, and D. T. Miller, Opt. Express 15, 16141 (2007). [CrossRef] [PubMed]
  9. K. Grieve and A. Roorda, Invest. Ophthalmol. Visual Sci. 49, 713 (2008). [CrossRef]
  10. G. Hanazono, K. Tsunoda, Y. Kazato, K. Tsubota, and M. Tanifuji, Invest. Ophthalmol. Visual Sci. 49, 4655 (2008). [CrossRef]
  11. V. J. Srinivasan, Y. Chen, J. S. Duker, and J. G. Fujimoto, Opt. Express 17, 3861 (2009). [CrossRef] [PubMed]
  12. X. C. Yao, A. Yamauchi, B. Perry, and J. S. George, Appl. Opt. 44, 2019 (2005). [CrossRef] [PubMed]
  13. K. Bizheva, R. Pflug, B. Hermann, B. Povazay, H. Sattmann, P. Qiu, E. Anger, H. Reitsamer, S. Popov, J. R. Taylor, A. Unterhuber, P. Ahnelt, and W. Drexler, Proc. Natl. Acad. Sci. USA 103, 5066 (2006). [CrossRef] [PubMed]
  14. Y. Chen, S. W. Huang, A. D. Aguirre, and J. G. Fujimoto, Opt. Lett. 32, 1971 (2007). [CrossRef] [PubMed]
  15. D. X. Hammer, R. D. Ferguson, T. E. Ustun, C. E. Bigelow, N. V. Iftimia, and R. H. Webb, J. Biomed. Opt. 11, 041126 (2006). [CrossRef] [PubMed]
  16. K. B. Im, S. Han, H. Park, D. Kim, and B. M. Kim, Opt. Express 13, 5151 (2005). [CrossRef] [PubMed]
  17. Q. V. Hoang, R. A. Linsenmeier, C. K. Chung, and C. A. Curcio, Visual Neurosci. 19, 395 (2002). [CrossRef]
  18. M. Kahlert, D. R. Pepperberg, and K. P. Hofmann, Nature 345, 537 (1990). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited