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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 3 — Feb. 1, 2010
  • pp: 447–449

Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging

Shan Shan Kou, Laura Waller, George Barbastathis, and Colin J.R. Sheppard  »View Author Affiliations

Optics Letters, Vol. 35, Issue 3, pp. 447-449 (2010)

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Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values.

© 2010 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: October 27, 2009
Revised Manuscript: December 16, 2009
Manuscript Accepted: December 20, 2009
Published: January 29, 2010

Virtual Issues
Vol. 5, Iss. 4 Virtual Journal for Biomedical Optics

Shan Shan Kou, Laura Waller, George Barbastathis, and Colin J. R. Sheppard, "Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging," Opt. Lett. 35, 447-449 (2010)

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