OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 35, Iss. 3 — Feb. 1, 2010
  • pp: 447–449

Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging

Shan Shan Kou, Laura Waller, George Barbastathis, and Colin J.R. Sheppard  »View Author Affiliations


Optics Letters, Vol. 35, Issue 3, pp. 447-449 (2010)
http://dx.doi.org/10.1364/OL.35.000447


View Full Text Article

Enhanced HTML    Acrobat PDF (220 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values.

© 2010 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval
(180.0180) Microscopy : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 27, 2009
Revised Manuscript: December 16, 2009
Manuscript Accepted: December 20, 2009
Published: January 29, 2010

Virtual Issues
Vol. 5, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Shan Shan Kou, Laura Waller, George Barbastathis, and Colin J. R. Sheppard, "Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging," Opt. Lett. 35, 447-449 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-3-447

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Supplementary Material


» Media 1: MOV (774 KB)     

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited