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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 5 — Mar. 1, 2010
  • pp: 781–783

Wavelength-coded multifocal microscopy

Yuan Luo, Se Baek Oh, and George Barbastathis  »View Author Affiliations

Optics Letters, Vol. 35, Issue 5, pp. 781-783 (2010)

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A wavelength-coded multifocal microscope incorporating multiplexed and wavelength-coded holographic gratings to generate wavelength-selective multifocal planes is presented. The focal planes are longitudinally spaced on the object plane, and each focal plane is probed by a designated wavelength. The recording of the multiplexed gratings takes place at a single wavelength by utilizing the Bragg degeneracy property; thus the maximum sensitive wavelength of blue 488 nm is used for recording, but the device is operated at a broad wavelength band of interest, all the way to red 633 nm . We present the design, implementation, and experimental image data demonstrating this microscope’s ability to obtain biological tissue structures simultaneously at different focal planes using broadband illumination by LEDs.

© 2010 Optical Society of America

OCIS Codes
(090.4220) Holography : Multiplex holography
(090.7330) Holography : Volume gratings
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: November 18, 2009
Revised Manuscript: January 24, 2010
Manuscript Accepted: January 27, 2010
Published: February 26, 2010

Virtual Issues
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics

Yuan Luo, Se Baek Oh, and George Barbastathis, "Wavelength-coded multifocal microscopy," Opt. Lett. 35, 781-783 (2010)

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