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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 6 — Mar. 15, 2010
  • pp: 886–888

Background suppression by axially selective activation in single-molecule localization microscopy

Danni Chen, Bin Yu, Junle Qu, and Hanben Niu  »View Author Affiliations

Optics Letters, Vol. 35, Issue 6, pp. 886-888 (2010)

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Resolution of a few tens of nanometers has been achieved in fluorescence microscopy with photoswitchable molecules. However, for thick samples, the background brought by the crosstalk of unwanted on-state molecules is nonnegligible. Now we present a background suppression method by using two axial standing waves generated by the interference of two activation beams with the same phases and two deactivation beams with the opposite phases. With spatially incoherent illumination, most activated molecules are located in a thin layer. The performance of such method is simulated with the known photoswitching characteristic of Cy5. With suitable parameters, the thickness of the layer can reach 39 nm (FWHM).

© 2010 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(180.6900) Microscopy : Three-dimensional microscopy
(170.2945) Medical optics and biotechnology : Illumination design

ToC Category:
Image Processing

Original Manuscript: November 10, 2009
Revised Manuscript: February 2, 2010
Manuscript Accepted: February 7, 2010
Published: March 15, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Danni Chen, Bin Yu, Junle Qu, and Hanben Niu, "Background suppression by axially selective activation in single-molecule localization microscopy," Opt. Lett. 35, 886-888 (2010)

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