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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 7 — Apr. 1, 2010
  • pp: 1028–1030

Phase microscopy of technical and biological samples through random phase modulation with a diffuser

Percival F. Almoro, Giancarlo Pedrini, Phanindra N. Gundu, Wolfgang Osten, and Steen G. Hanson  »View Author Affiliations

Optics Letters, Vol. 35, Issue 7, pp. 1028-1030 (2010)

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A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method.

© 2010 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(060.5060) Fiber optics and optical communications : Phase modulation
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(230.1980) Optical devices : Diffusers

ToC Category:
Image Processing

Original Manuscript: December 10, 2009
Revised Manuscript: February 1, 2010
Manuscript Accepted: February 12, 2010
Published: March 30, 2010

Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics

Percival F. Almoro, Giancarlo Pedrini, Phanindra N. Gundu, Wolfgang Osten, and Steen G. Hanson, "Phase microscopy of technical and biological samples through random phase modulation with a diffuser," Opt. Lett. 35, 1028-1030 (2010)

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