A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.
© 2010 Optical Society of America
Original Manuscript: January 4, 2010
Revised Manuscript: March 11, 2010
Manuscript Accepted: March 12, 2010
Published: April 15, 2010
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics
Ivo M. Vellekoop and Christof M. Aegerter, "Scattered light fluorescence microscopy: imaging through turbid layers," Opt. Lett. 35, 1245-1247 (2010)