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Optics Letters

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  • Vol. 36, Iss. 1 — Jan. 1, 2011
  • pp: 40–42

Rotation of single bacterial cells relative to the optical axis using optical tweezers

G. Carmon and M. Feingold  »View Author Affiliations


Optics Letters, Vol. 36, Issue 1, pp. 40-42 (2011)
http://dx.doi.org/10.1364/OL.36.000040


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Abstract

Using a single-beam, oscillating optical tweezers, we demonstrate trapping and rotation of rod-shaped bacterial cells with respect to the optical axis. The angle of rotation, θ, is determined by the amplitude of the oscillation. It is shown that θ can be measured from the longitudinal cell intensity profiles in the corresponding phase-contrast images. The technique allows viewing the cell from different perspectives and can provide a useful tool in fluorescence microscopy for the analysis of three-dimensional subcellular structures.

© 2011 Optical Society of America

OCIS Codes
(110.2960) Imaging systems : Image analysis
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 17, 2010
Revised Manuscript: November 11, 2010
Manuscript Accepted: November 14, 2010
Published: December 21, 2010

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Citation
G. Carmon and M. Feingold, "Rotation of single bacterial cells relative to the optical axis using optical tweezers," Opt. Lett. 36, 40-42 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-1-40


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