Using a single-beam, oscillating optical tweezers, we demonstrate trapping and rotation of rod-shaped bacterial cells with respect to the optical axis. The angle of rotation, θ, is determined by the amplitude of the oscillation. It is shown that θ can be measured from the longitudinal cell intensity profiles in the corresponding phase-contrast images. The technique allows viewing the cell from different perspectives and can provide a useful tool in fluorescence microscopy for the analysis of three-dimensional subcellular structures.
© 2011 Optical Society of America
Original Manuscript: August 17, 2010
Revised Manuscript: November 11, 2010
Manuscript Accepted: November 14, 2010
Published: December 21, 2010
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics
G. Carmon and M. Feingold, "Rotation of single bacterial cells relative to the optical axis using optical tweezers," Opt. Lett. 36, 40-42 (2011)